http://scholars.ntou.edu.tw/handle/123456789/17741
標題: | Differential effects of SUMO1 and SUMO2 on circadian protein PER2 stability and function | 作者: | Chen, Ling-Chih Hsieh, Yung-Lin Tan, Grace Y. T. Kuo, Tai-Yun Chou, Yu-Chi Hsu, Pang-Hung Hwang-Verslues, Wendy W. |
公開日期: | 13-七月-2021 | 出版社: | NATURE RESEARCH | 卷: | 11 | 期: | 1 | 來源出版物: | SCIENTIFIC REPORTS | 摘要: | Posttranslational modification (PTM) of core circadian clock proteins, including Period2 (PER2), is required for proper circadian regulation. PER2 function is regulated by casein kinase 1 (CK1)-mediated phosphorylation and ubiquitination but little is known about other PER2 PTMs or their interaction with PER2 phosphorylation. We found that PER2 can be SUMOylated by both SUMO1 and SUMO2; however, SUMO1 versus SUMO2 conjugation had different effects on PER2 turnover and transcriptional suppressor function. SUMO2 conjugation facilitated PER2 interaction with beta -TrCP leading to PER2 proteasomal degradation. In contrast, SUMO1 conjugation, mediated by E3 SUMO-protein ligase RanBP2, enhanced CK1-mediated PER2(S662) phosphorylation, inhibited PER2 degradation and increased PER2 transcriptional suppressor function. PER2 K736 was critical for both SUMO1- and SUMO2-conjugation. A PER2(K736R) mutation was sufficient to alter PER2 protein oscillation and reduce PER2-mediated transcriptional suppression. Together, our data revealed that SUMO1 versus SUMO2 conjugation acts as a determinant of PER2 stability and function and thereby affects the circadian regulatory system and the expression of clock-controlled genes. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/17741 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-021-93933-y |
顯示於: | 生命科學暨生物科技學系 |
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