|Title:||AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms||Authors:||Te-Hua Hsu
|Keywords:||AFLP;SNPs;Molecular Marker;SSCP||Issue Date:||1-Oct-2014||Journal Volume:||4||Journal Issue:||6||Start page/Pages:||376 - 381||Source:||Advances in Biological Chemistry||Abstract:||
Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present a cheap and convenient AFLPbased (Amplified fragment length polymorphism) strategy that is highly efficient for developing informative SNP markers without any prior information. We developed SNP markers in a nonmodel and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential use of the combinations of AFLP and AFLP-SSCP.
|Appears in Collections:||水產養殖學系|
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