http://scholars.ntou.edu.tw/handle/123456789/20705
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Fonseca, Alexis | en_US |
dc.contributor.author | Ishoey, Thomas | en_US |
dc.contributor.author | Carole Espinoza | en_US |
dc.contributor.author | Perez-Pantoja, Danilo | en_US |
dc.contributor.author | Manghisi, Antonio | en_US |
dc.contributor.author | Morabito, Marina | en_US |
dc.contributor.author | Salas-Burgos, Alexis | en_US |
dc.contributor.author | Gallardo, Victor A. | en_US |
dc.date.accessioned | 2022-02-17T05:27:48Z | - |
dc.date.available | 2022-02-17T05:27:48Z | - |
dc.date.issued | 2017-12-13 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/20705 | - |
dc.description.abstract | The Humboldt Sulfuretum (HS), in the productive Humboldt Eastern Boundary Current Upwelling Ecosystem, extends under the hypoxic waters of the Peru-Chile Undercurrent (ca. 6 degrees S and ca. 36 degrees S). Studies show that primeval sulfuretums held diverse prokaryotic life, and, while rare today, still sustain species-rich giant sulfur-oxidizing bacterial communities. We here present the genomic features of a new bacteria of the HS, Candidatus Venteria ishoeyi (Ca. V. ishoeyi) in the family Thiotrichaceae. Three identical filaments were micromanipulated from reduced sediments collected off central Chile; their DNA was extracted, amplified, and sequenced by a Roche 454 GS FLX platform. Using three sequenced libraries and through de novo genome assembly, a draft genome of 5.7 Mbp, 495 scaffolds, and a N50 of 70 kbp, was obtained. The 16S rRNA gene phylogenetic analysis showed that Ca. V. ishoeyi is related to non-vacuolate forms presently known as Beggiatoa or Beggiatoa-like forms. The complete set of genes involved in respiratory nitrate-reduction to dinitrogen was identified in Ca. V. ishoeyi; including genes likely leading to ammonification. As expected, the sulfur-oxidation pathway reported for other sulfur-oxidizing bacteria were deduced and also, key inorganic and organic carbon acquisition related genes were identified. Unexpectedly, the genome of Ca. V. ishoeyi contained numerous CRISPR repeats and an I-F CRISPR-Cas type system gene coding array. Findings further show that, as a member of an eons-old marine ecosystem, Ca. V. ishoeyi contains the needed metabolic plasticity for life in an increasingly oxygenated and variable ocean. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PUBLIC LIBRARY SCIENCE | en_US |
dc.relation.ispartof | PLOS ONE | en_US |
dc.subject | ELECTRON-TRANSPORT PATHWAYS | en_US |
dc.subject | C NITRITE REDUCTASE | en_US |
dc.subject | SULFIDE OXIDATION | en_US |
dc.subject | THIOPLOCA-ARAUCAE | en_US |
dc.subject | SP-NOV | en_US |
dc.subject | BEGGIATOA | en_US |
dc.subject | NITRATE | en_US |
dc.subject | BACTERIA | en_US |
dc.subject | MARINE | en_US |
dc.subject | 16S | en_US |
dc.title | Genomic features of Candidatus Venteria ishoeyi, a new sulfur-oxidizing macrobacterium from the Humboldt Sulfuretum off Chile | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1371/journal.pone.0188371 | - |
dc.identifier.isi | WOS:000417884100016 | - |
dc.relation.journalvolume | 12 | en_US |
dc.relation.journalissue | 12 | en_US |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
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