http://scholars.ntou.edu.tw/handle/123456789/20705
Title: | Genomic features of Candidatus Venteria ishoeyi, a new sulfur-oxidizing macrobacterium from the Humboldt Sulfuretum off Chile | Authors: | Fonseca, Alexis Ishoey, Thomas Carole Espinoza Perez-Pantoja, Danilo Manghisi, Antonio Morabito, Marina Salas-Burgos, Alexis Gallardo, Victor A. |
Keywords: | ELECTRON-TRANSPORT PATHWAYS;C NITRITE REDUCTASE;SULFIDE OXIDATION;THIOPLOCA-ARAUCAE;SP-NOV;BEGGIATOA;NITRATE;BACTERIA;MARINE;16S | Issue Date: | 13-Dec-2017 | Publisher: | PUBLIC LIBRARY SCIENCE | Journal Volume: | 12 | Journal Issue: | 12 | Source: | PLOS ONE | Abstract: | The Humboldt Sulfuretum (HS), in the productive Humboldt Eastern Boundary Current Upwelling Ecosystem, extends under the hypoxic waters of the Peru-Chile Undercurrent (ca. 6 degrees S and ca. 36 degrees S). Studies show that primeval sulfuretums held diverse prokaryotic life, and, while rare today, still sustain species-rich giant sulfur-oxidizing bacterial communities. We here present the genomic features of a new bacteria of the HS, Candidatus Venteria ishoeyi (Ca. V. ishoeyi) in the family Thiotrichaceae. Three identical filaments were micromanipulated from reduced sediments collected off central Chile; their DNA was extracted, amplified, and sequenced by a Roche 454 GS FLX platform. Using three sequenced libraries and through de novo genome assembly, a draft genome of 5.7 Mbp, 495 scaffolds, and a N50 of 70 kbp, was obtained. The 16S rRNA gene phylogenetic analysis showed that Ca. V. ishoeyi is related to non-vacuolate forms presently known as Beggiatoa or Beggiatoa-like forms. The complete set of genes involved in respiratory nitrate-reduction to dinitrogen was identified in Ca. V. ishoeyi; including genes likely leading to ammonification. As expected, the sulfur-oxidation pathway reported for other sulfur-oxidizing bacteria were deduced and also, key inorganic and organic carbon acquisition related genes were identified. Unexpectedly, the genome of Ca. V. ishoeyi contained numerous CRISPR repeats and an I-F CRISPR-Cas type system gene coding array. Findings further show that, as a member of an eons-old marine ecosystem, Ca. V. ishoeyi contains the needed metabolic plasticity for life in an increasingly oxygenated and variable ocean. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20705 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0188371 |
Appears in Collections: | 14 LIFE BELOW WATER 15 LIFE ON LAND |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.