http://scholars.ntou.edu.tw/handle/123456789/23317
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Winton Cheng | en_US |
dc.contributor.author | Ying-Hsiu Tung | en_US |
dc.contributor.author | Chun-Hung Liu | en_US |
dc.contributor.author | Jiann-Chu Chen | en_US |
dc.date.accessioned | 2022-12-07T07:54:46Z | - |
dc.date.available | 2022-12-07T07:54:46Z | - |
dc.date.issued | 2006-04 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/23317 | - |
dc.description.abstract | A cytosolic manganese superoxide dismutase (cytMn-SOD) cDNA was cloned from the hepatopancreas of giant freshwater prawn Macrobrachium rosenbergii using reverse transcription polymerase chain reaction (RT-PCR) by degenerate primers. Both 3'- and 5'-regions were isolated by rapid amplification of cDNA end RACE method. Analysis of nucleotide sequence revealed that the cytMn-SOD cDNA clone consists of 1339 bp with an open reading frame of 858 bp encoding a protein of 286 amino acids. The calculated molecular mass of the mature proteins (286 amino acids) is 31 kDa with an estimated pI of 5.52. Two putative N-glycosylation sites, NXT and NXS were observed in the cytMn-SOD. Four conserved amino acids responsible for binding manganese were observed (H 110, H 158, D243 and H247). Sequence comparison showed that the cytMn-SOD deduced amino acid sequence of M. rosenbergii has an overall similarity of 77% and 54% to that of blue crab Callinectes sapidus and tiger shrimp Penaeus monodon, respectively. Quantitative real-time RT-PCR analysis showed that cytMn-SOD transcript in hepatopancreas of M. rosenbergii decreased 3 h after Lactococcus garvieae injection, but no significant change in cytMn-SOD transcript was observed in the haemocytes 3-24 h after L. garvieae injection. (C) 2005 Elsevier Ltd. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartof | FISH & SHELLFISH IMMUNOLOGY | en_US |
dc.subject | POLYMERASE-CHAIN-REACTION | en_US |
dc.subject | LACTOCOCCUS-GARVIEAE | en_US |
dc.subject | IMMUNE RESISTANCE | en_US |
dc.subject | SUSCEPTIBILITY | en_US |
dc.subject | CRUSTACEANS | en_US |
dc.subject | SHRIMP | en_US |
dc.subject | TEMPERATURE | en_US |
dc.subject | HEMOCYTES | en_US |
dc.subject | ENZYMES | en_US |
dc.subject | STRESS | en_US |
dc.title | Molecular cloning and characterisation of cytosolic manganese superoxide dismutase (cytMn-SOD) from the giant freshwater prawn Macrobrachium rosenbergii | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.fsi.2005.05.016 | - |
dc.identifier.isi | WOS:000234165800001 | - |
dc.relation.journalvolume | 20 | en_US |
dc.relation.journalissue | 4 | en_US |
dc.relation.pages | 438-449 | en_US |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
顯示於: | 水產養殖學系 |
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