Impacts of polystyrene nanoplastics on microgel formation from effluent organic matter

Abstract

Municipal effluents discharged from wastewater treatment plants (WWTPs) are considered major contributors of nanoplastics (NPs) and dissolved effluent organic matter (dEfOM) to environments. Due to their small sizes, NPs can travel easily in waterways and evade wastewater treatment processes, and may directly interact with dEfOM, altering their environmental fates. However, although much research has examined the impact of natural organic matter on NPs, the interactions between NPs and dEfOM remain unexplored. This study investigated the influences of NPs on the behavior and capacity of dEfOM aggregation and surface granularity, and identified the possible aggregation mechanism. We also adjusted the salinity of water samples to simulate scenarios based on WWTP-sea continuums. Our data suggest that dEfOM can self-assemble with 55 nm polystyrene NPs to form microgels, particularly under high salinity conditions. NPs accelerates the formation speed and number of dEfOM aggregates, but the sizes of the aggregates remain largely unchanged. The relative particle counts at a salinity of 34 psu increased by 300 % compared to the control group. The potential mechanism behind NPs-microgels aggregation is likely driven by the synergistic effect of the divalent ion crosslinking and hydrophobic interactions between EfOM and NPs. Notably, NPs incorporation into microgels decreases the surface granularity, thereby possibly affecting settling velocity and colonization of aggregates, as well as microbial attachment and community diversity. Overall, our findings demonstrate the potential influence of NPs on dEfOM assembly and surface properties following effluent discharge, and can inspire further relevant studies on microorganism interactions, removal technologies, and the environmental transport of NPs.