http://scholars.ntou.edu.tw/handle/123456789/3715
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hung, Lien-Yu | en_US |
dc.contributor.author | Fu, Chien-Yu | en_US |
dc.contributor.author | Wang, Chih-Hung | en_US |
dc.contributor.author | Chuang, Yuan-Jhe | en_US |
dc.contributor.author | Tsai, Yi-Cheng | en_US |
dc.contributor.author | Lo, Yi-Ling | en_US |
dc.contributor.author | Hsu, Pang-Hung | en_US |
dc.contributor.author | Chang, Hwan-You | en_US |
dc.contributor.author | Shiesh, Shu-Chu | en_US |
dc.contributor.author | Hsu, Keng-Fu | en_US |
dc.contributor.author | Lee, Gwo-Bin | en_US |
dc.date.accessioned | 2020-11-18T09:30:04Z | - |
dc.date.available | 2020-11-18T09:30:04Z | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3715 | - |
dc.description.abstract | Cancer is the most serious disease worldwide, and ovarian cancer (OvCa) is the second most common type of gynecological cancer. There is consequently an urgent need for early-stage detection of OvCa, which requires affinity reagent biomarkers for OvCa. Systematic evolution of ligands by exponential enrichment (SELEX) and phage display technology are two powerful technologies for identifying affinity reagent biomarkers. However, the benchtop protocols for both screening technologies are relatively lengthy and require well-trained personnel. We therefore developed a novel, integrated microfluidic system capable of automating SELEX and phage display technology. Instead of using cancer cell lines, it is the first work which used tissue slides as screening targets, which possess more complicated and uncovered information for affinity reagents to recognize. This allowed for the identification of aptamer (nucleic acid) and peptide probes specific to OvCa cells and tissues. Furthermore, this developed system could be readily modified to uncover affinity reagents for diagnostics or even target therapy of other cancer cell types in the future. Published by AIP Publishing. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER INST PHYSICS | en_US |
dc.relation.ispartof | BIOMICROFLUIDICS | en_US |
dc.subject | CHIP CELL-SELEX | en_US |
dc.subject | OVARIAN-CANCER | en_US |
dc.subject | APTAMERS | en_US |
dc.subject | PHAGE | en_US |
dc.subject | STATISTICS | en_US |
dc.subject | DIAGNOSIS | en_US |
dc.subject | SELECTION | en_US |
dc.subject | IDENTIFICATION | en_US |
dc.subject | SYSTEM | en_US |
dc.subject | BRCA1 | en_US |
dc.title | Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1063/1.5050451 | - |
dc.identifier.isi | WOS:000448954600011 | - |
dc.identifier.url | <Go to ISI>://WOS:000448954600011 | |
dc.relation.journalvolume | 12 | en_US |
dc.relation.journalissue | 5 | en_US |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.orcid | 0000-0001-6873-6434 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 海洋生物科技學士學位學程(系) 生命科學暨生物科技學系 03 GOOD HEALTH AND WELL-BEING |
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