http://scholars.ntou.edu.tw/handle/123456789/3760| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Young-Mao Chen | en_US |
| dc.contributor.author | Kuo, Cham-En | en_US |
| dc.contributor.author | Wang, Ting-Yu | en_US |
| dc.contributor.author | Shie, Pei-Shiuan | en_US |
| dc.contributor.author | Wang, Wei-Chen | en_US |
| dc.contributor.author | Huang, Shao-Ling | en_US |
| dc.contributor.author | Tsai, Tieh-Jung | en_US |
| dc.contributor.author | Chen, Peng-Peng | en_US |
| dc.contributor.author | Jiann-Chu Chen | en_US |
| dc.contributor.author | Tzong-Yueh Chen | en_US |
| dc.date.accessioned | 2020-11-18T09:46:05Z | - |
| dc.date.available | 2020-11-18T09:46:05Z | - |
| dc.date.issued | 2010-05 | - |
| dc.identifier.issn | 1050-4648 | - |
| dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3760 | - |
| dc.description.abstract | The heat shock proteins (HSPs) family which consists of HSP90, HSP70, and low molecular mass HSPs are involved in chaperone activity. Here, we report the cloning and characterization of HSP90AB gene from orange-spotted grouper, Epinephelus coioides. The full-length of grouper HSP90AB was 727 amino acids and possessed an ATPase domain as well as an evolutionarily conserved molecular chaperone. The HSP90AB-green fluorescent protein fusion protein was evenly distributed in the cytoplasm. Immunohistochemistry (IHC) and real-time polymerase chain reaction (PCR) analyses indicated that the expression of grouper HSP90AB was marginally increased following nodavirus infection. Grouper E. coioides that received HSP90 inhibitor geldanamycin (GA) showed an increase in HSP90AB expression and growth of nodavirus supporting nodavirus replication. (C) 2010 Elsevier Ltd. All rights reserved. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | ELSEVIER | en_US |
| dc.relation.ispartof | Fish & Shellfish Immunology | en_US |
| dc.subject | OXIDATIVE STRESS | en_US |
| dc.subject | GENE-EXPRESSION | en_US |
| dc.subject | RNA | en_US |
| dc.subject | CHAPERONE | en_US |
| dc.subject | CDNA | en_US |
| dc.subject | CELLS | en_US |
| dc.subject | REPLICATION | en_US |
| dc.subject | ACTIVATION | en_US |
| dc.subject | INDUCTION | en_US |
| dc.subject | INFECTION | en_US |
| dc.title | Cloning of an orange-spotted grouper Epinephelus coioides heat shock protein 90AB (HSP90AB) and characterization of its expression in response to nodavirus | en_US |
| dc.type | journal article | en_US |
| dc.identifier.doi | 10.1016/j.fsi.2010.02.004 | - |
| dc.identifier.isi | WOS:000277822100020 | - |
| dc.relation.journalvolume | 28 | en_US |
| dc.relation.journalissue | 5-6 | en_US |
| dc.relation.pages | 895-904 | en_US |
| item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
| item.cerifentitytype | Publications | - |
| item.languageiso639-1 | en_US | - |
| item.fulltext | no fulltext | - |
| item.grantfulltext | none | - |
| item.openairetype | journal article | - |
| crisitem.author.dept | College of Life Sciences | - |
| crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
| crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
| crisitem.author.dept | Department of Aquaculture | - |
| crisitem.author.dept | College of Life Sciences | - |
| crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
| crisitem.author.dept | College of Ocean Science and Resource | - |
| crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
| crisitem.author.dept | Institute of Marine Environment and Ecology | - |
| crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
| crisitem.author.parentorg | College of Life Sciences | - |
| crisitem.author.parentorg | College of Life Sciences | - |
| crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
| crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
| crisitem.author.parentorg | College of Ocean Science and Resource | - |
| 顯示於： | 海洋生物科技學士學位學程(系) | |
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