http://scholars.ntou.edu.tw/handle/123456789/3796
標題: | Nested-PCR and TaqMan real-time quantitative PCR assays for human adenoviruses in environmental waters | 作者: | Wen-Chien Huang Yi-Pen Chou Po-Min Kao Tsui-Kang Hsu Hung-Chang Su Ying-Ning Ho Yi-Chun Yang Bing-Mu Hsu |
關鍵字: | conductivity;human adenovirus;heterotrophic plate count;qPCR;river water | 公開日期: | 四月-2016 | 出版社: | IWA Publishing | 卷: | 73 | 期: | 8 | 起(迄)頁: | 1832–1841 | 來源出版物: | Water Science and Technology | 摘要: | Human adenovirus (HAdV) infections can occur throughout the year. Cases of HAdV-associated respiratory disease have been more common in the late winter, spring, and early summer. In this study, to provide viral pollution data for further epidemiological studies and governmental actions, the presence of HAdV in the aquatic environment was quantitatively surveyed in the summer. This study was conducted to compare the efficiencies of nested-PCR (polymerase chain reaction) and qPCR (quantitative PCR) for detecting HAdV in environmental waters. A total of 73 water samples were collected from Puzi River in Taiwan and subjected to virus concentration methods. In the results, qPCR had much better efficiency for specifying the pathogen in river sample. HAdV41 was detected most frequently in the river water sample (10.9%). The estimated HAdV concentrations ranged between 6.75 × 102 and 2.04 × 109 genome copies/L. Significant difference was also found in heterotrophic plate counts, conductivity, water temperature, and water turbidity between presence/absence of HAdV. HAdV in the Puzi River may pose a significant health risk. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/3796 | ISSN: | 0273-1223 | DOI: | 10.2166/wst.2016.004 |
顯示於: | 海洋生物研究所 |
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