http://scholars.ntou.edu.tw/handle/123456789/5057
標題: | Multi-residue analysis using liquid chromatography tandem mass spectrometry for detection of 20 coccidiostats in poultry, livestock, and aquatic tissues | 作者: | Shun-Hsien Chang Yu-Hsun Lai Chih-Neng Huang Guan-Jhih Peng Chia-Ding Liao Ya-Min Kao Su-HsiangTseng Der-Yuan Wang |
關鍵字: | Coccidiostats;LC–MS/MS;Multi-residue analysis;QuEChERS;Veterinary drugs | 公開日期: | 25-二月-2019 | 出版社: | ScienceDirect | 卷: | 27 | 期: | 3 | 起(迄)頁: | 703-716 | 來源出版物: | Journal of Food and Drug Analysis | 摘要: | In this study, we developed a novel analysis method based on liquid chromatography/tandem mass spectrometry (LC–MS/MS) to allow the simultaneous identification of 20 coccidiostats in eight matrix categories, including the muscles of chicken, swine, cow, and fish as well as chicken eggs, bovine milk, and porcine viscera. In the pretreatment procedure, acetonitrile/methanol (95:5, v/v) containing 1% formic acid, 5 g of sodium acetate, and 6.0 g of anhydrous magnesium sulfate was used for extraction, followed by a clean-up procedure using n-hexane saturated with ACN to facilitate the elimination of analytes from high lipid samples. Chromatographic separations were achieved using a Poroshell 120SB C18 column and operated with a gradient mobile phase system consisting of methanol (with 0.1% formic acid) and 5 mM ammonium formate, and the MS detection was monitored simultaneously. The method was validated in accordance with the Guidelines for the Validation of Food Chemical Methods by the Taiwan Food and Drug Administration. The limit of quantitation among 8 matrices were 0.5–2 ng g−1. The proposed method proved highly effective in detecting the presence of targeted veterinary drugs, providing a high degree of precision and accuracy over a broad range of matrices. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/5057 | ISSN: | 1021-9498 | DOI: | 10.1016/j.jfda.2019.02.004 |
顯示於: | 食品安全與風險管理研究所 |
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