http://scholars.ntou.edu.tw/handle/123456789/6095
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Tsung-Meng Wu | en_US |
dc.contributor.author | Fan-Hua Nan | en_US |
dc.contributor.author | Kuan-Chu Chen | en_US |
dc.contributor.author | Yu-Sheng Wu | en_US |
dc.date.accessioned | 2020-11-20T00:40:30Z | - |
dc.date.available | 2020-11-20T00:40:30Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/6095 | - |
dc.description.abstract | In this study, the effects of acetyl-xylogalactan extracted from Sarcodia suieae on RAW 264.7 macrophage polarisation were evaluated. This extracted acetyl-xylogalactan had a monosaccharide composition of 91% galactose and 9% xylose, with polysaccharide and acetyl contents of 80.6% and 19.3%, respectively. MALDI–TOF mass spectrometry and NMR spectroscopy revealed the molecular weight of the acetyl-xylogalactan to be 88.5 kDa. After acetyl-xylogalactan treatment, RAW 264.7 macrophage polarisation was noted, along with enhanced phagocytic ability. Furthermore, the Cell Counting Kit-8 (CCK-8) assay was performed and the results demonstrated non-significant alteration in lactate dehydrogenase levels in the treated cells. Next, interleukin (IL) 1β, TNF, and Malt-1 expression in RAW 264.7 macrophages treated with the S. suieae acetyl-xylogalactan was investigated through real-time quantitative polymerase chain reaction, and the results demonstrated that S. suieae acetyl-xylogalactan induced IL-1β and Malt-1 expression. RNA sequencing analysis results indicated the S. suieae acetyl-xylogalactan positively regulated cytokine production and secretion, protein secretion, and response to IL-1 activation, based on the observed GO terms. The predicted target genes in the GO enrichment analysis were found to upregulate NF-κB signalling and M0 to M1 macrophage conversion through the observed cytokine production. Thus, acetyl-xylogalactan can positively regulate RAW 264.7 macrophage polarisation. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Scientific Reports | en_US |
dc.title | Sarcodia suieae acetyl-xylogalactan regulate RAW 264.7 macrophage NF-kappa B activation and IL-1 beta cytokine production in macrophage polarization | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1038/s41598-019-56246-9 | - |
dc.identifier.isi | WOS:000508896900018 | - |
dc.relation.journalvolume | 9 | en_US |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.orcid | 0000-0003-4133-7171 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 水產養殖學系 |
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