http://scholars.ntou.edu.tw/handle/123456789/3828
標題: | The novel white spot syndrome virus-induced gene, PmERP15, encodes an ER stress-responsive protein in black tiger shrimp, Penaeus monodon | 作者: | Jiann-Horng Leu Kuan-Fu Liu Kuan-Yu Chen Shu-Hwa Chen Yu-Bin Wang Chung-Yen Lin Chu-Fang Lo |
關鍵字: | White spot syndrome virus;Penaeus monodon;ER stress;Unfolded protein response | 公開日期: | 四月-2015 | 出版社: | ELSEVLER | 卷: | 49 | 期: | 2 | 起(迄)頁: | 239-248 | 來源出版物: | Developmental and Comparative Immunology | 摘要: | By microarray screening, we identified a white spot syndrome virus (WSSV)-strongly induced novel gene in gills of Penaeus monodon. The gene, PmERP15, encodes a putative transmembrane protein of 15 kDa, which only showed some degree of similarity (54–59%) to several unknown insect proteins, but had no hits to shrimp proteins. RT-PCR showed that PmERP15 was highly expressed in the hemocytes, heart and lymphoid organs, and that WSSV-induced strong expression of PmERP15 was evident in all tissues examined. Western blot analysis likewise showed that WSSV strongly up-regulated PmERP15 protein levels. In WSSV-infected hemocytes, immunofluorescence staining showed that PmERP15 protein was colocalized with an ER enzyme, protein disulfide isomerase, and in Sf9 insect cells, PmERP15-EGFP fusion protein colocalized with ER -Tracker™ Red dye as well. GRP78, an ER stress marker, was found to be up-regulated in WSSV-infected P. monodon, and both PmERP15 and GRP78 were up-regulated in shrimp injected with ER stress inducers tunicamycin and dithiothreitol. Silencing experiments showed that although PmERP15 dsRNA-injected shrimp succumbed to WSSV infection more rapidly, the WSSV copy number had no significant changes. These results suggest that PmERP15 is an ER stress-induced, ER resident protein, and its induction in WSSV-infected shrimp is caused by the ER stress triggered by WSSV infection. Furthermore, although PmERP15 has no role in WSSV multiplication, its presence is essential for the survival of WSSV-infected shrimp. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/3828 | ISSN: | 0145-305X | DOI: | 10.1016/j.dci.2014.12.001 |
顯示於: | 海洋生物研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。