http://scholars.ntou.edu.tw/handle/123456789/5335
標題: | Integrated Hypoxia Signaling and Oxidative Stress in Developmental Neurotoxicity of Benzo[a]Pyrene in Zebrafish Embryos | 作者: | Lin, Yi-Chen Wu, Chang-Yi Hu, Chin-Hwa Pai, Tun-Wen Chen, Yet-Ran Wang, Wen-Der |
關鍵字: | POLYCYCLIC AROMATIC-HYDROCARBONS;EARLY ADOLESCENCE PERIOD;HYDROGEN-PEROXIDE;LIPID-PEROXIDATION;NEURAL INDUCTION;RETINOIC ACID;DNA-DAMAGE;EXPRESSION;GENE;EXPOSURE | 公開日期: | 八月-2020 | 出版社: | MDPI | 卷: | 9 | 期: | 8 | 來源出版物: | ANTIOXIDANTS-BASEL | 摘要: | Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon formed by the incomplete combustion of organic matter. Environmental B[a]P contamination poses a serious health risk to many organisms because the pollutant may negatively affect many physiological systems. As such, chronic exposure to B[a]P is known to lead to locomotor dysfunction and neurodegeneration in several organisms. In this study, we used the zebrafish model to delineate the acute toxic effects of B[a]P on the developing nervous system. We found that embryonic exposure of B[a]P downregulatesshhandisl1, causing morphological hypoplasia in the telencephalon, ventral thalamus, hypothalamus, epiphysis and posterior commissure. Moreover, hypoxia-inducible factors (hif1aandhif2a) are repressed upon embryonic exposure of B[a]P, leading to reduced expression of the Hif-target genes,epoandsurvivin, which are associated with neural differentiation and maintenance. During normal embryogenesis, low-level oxidative stress regulates neuronal development and function. However, our experiments revealed that embryonic oxidative stress is greatly increased in B[a]P-treated embryos. The expression ofcatalasewas decreased andsod1expression increased in B[a]P-treated embryos. These transcriptional changes were coincident with increased embryonic levels of H(2)O(2)and malondialdehyde, with the levels in B[a]P-treated fish similar to those in embryos treated with 120-mu M H2O2. Together, our data suggest that reduced Hif signaling and increased oxidative stress are involved in B[a]P-induced acute neurotoxicity during embryogenesis. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/5335 | ISSN: | 2076-3921 | DOI: | 10.3390/antiox9080731 |
顯示於: | 生命科學暨生物科技學系 03 GOOD HEALTH AND WELL-BEING 資訊工程學系 11 SUSTAINABLE CITIES & COMMUNITIES |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。