http://scholars.ntou.edu.tw/handle/123456789/5894
Title: | Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway | Authors: | Chen, Cheng-Hsien Sue, Yuh-Mou Cheng, Chung-Yi Chen, Yen-Cheng Liu, Chung-Te Hsu, Yung-Ho Hwang, Pai-An Huang, Nai-Jen Chen, Tso-Hsiao |
Keywords: | MOLECULAR-WEIGHT FUCOIDAN;OBSTRUCTIVE NEPHROPATHY;IN-VIVO;ISCHEMIA/REPERFUSION INJURY;SULFATED POLYSACCHARIDES;MESENCHYMAL TRANSITION;INTERSTITIAL FIBROSIS;DIABETIC-NEPHROPATHY;URETERAL OBSTRUCTION;UNDARIA-PINNATIFIDA | Issue Date: | 18-Jan-2017 | Publisher: | NATURE PUBLISHING GROUP | Journal Volume: | 7 | Source: | SCI REP-UK | Abstract: | Tubulointerstitial fibrosis is recognized as a key determinant of progressive chronic kidney disease (CKD). Fucoidan, a sulphated polysaccharide extracted from brown seaweed, exerts beneficial effects in some nephropathy models. The present study evaluated the inhibitory effect of oligo-fucoidan (800 Da) on renal tubulointerstitial fibrosis. We established a mouse CKD model by right nephrectomy with transient ischemic injury to the left kidney. Six weeks after the surgery, we fed the CKD mice oligo-fucoidan at 10, 20, and 100 mg/kg/d for 6 weeks and found that the oligo-fucoidan doses less than 100 mg/kg/d improved renal function and reduced renal tubulointerstitial fibrosis in CKD mice. Oligo-fucoidan also inhibited pressure-induced fibrotic responses and the expression of CD44, beta-catenin, and TGF-beta in rat renal tubular cells (NRK-52E). CD44 knockdown downregulated the expression of beta-catenin and TGF-beta in pressure-treated cells. Additional ligands for CD44 reduced the anti-fibrotic effect of oligo-fucoidan in NRK-52E cells. These data suggest that oligo-fucoidan at the particular dose prevents renal tubulointerstitial fibrosis in a CKD model. The anti-fibrotic effect of oligo-fucoidan may result from interfering with the interaction between CD44 and its extracellular ligands. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/5894 | ISSN: | 2045-2322 | DOI: | 10.1038/srep40183 |
Appears in Collections: | 生命科學暨生物科技學系 03 GOOD HEALTH AND WELL-BEING |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.