http://scholars.ntou.edu.tw/handle/123456789/5899
標題: | Taurine resumed neuronal differentiation in arsenite-treated N2a cells through reducing oxidative stress, endoplasmic reticulum stress, and mitochondrial dysfunction | 作者: | Chien-Te Chou Hong-Ting Lin Pai-An Hwang Shang-Ta Wang Cheng-Hong Hsieh Deng-Fwu Hwang |
關鍵字: | Arsenite;Neuronal differentiation;Taurine;ER stress;Oxidative stress | 公開日期: | 十二月-2014 | 出版社: | Springer | 卷: | 47 | 期: | 4 | 起(迄)頁: | 735-744 | 來源出版物: | Amino Acids | 摘要: | The goal of the study is to investigate the preventive effect of taurine against arsenite-induced arrest of neuronal differentiation in N2a cells. Our results revealed that taurine reinstated the neurite outgrowth in arsenite-treated N2a cells. Meanwhile, arsenite-induced oxidative stress and mitochondrial dysfunction as well as degradation of mitochondria DNA (mtDNA) were also inhibited by co-treatment of taurine. Since oxidative stress and mitochondrial dysfunction is closely associated with endoplasmic reticulum (ER) stress, we further examined indicators of ER stress, 78 kDa glucose-regulated protein (GRP78), and C/EBP-homologous protein (CHOP) protein expression. The results demonstrated that taurine significantly reduced arsenite-induced ER stress in N2a cells. In the parallel experiment, arsenite-induced disruption of intracellular calcium homeostasis was also ameliorated by taurine. The proven bio-function of taurine preserved a preventive effect against deleteriously cross-talking between oxidative stress, mitochondria, and ER. Overall, the results of the study suggested that taurine reinstated neuronal differentiation by inhibiting oxidative stress, ER stress, and mitochondrial dysfunction in arsenite-treated N2a cells. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/5899 | ISSN: | 0939-4451 | DOI: | 10.1007/s00726-014-1901-1 |
顯示於: | 生命科學暨生物科技學系 |
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