http://scholars.ntou.edu.tw/handle/123456789/9749
標題: | Identification of species and measurement of tetrodotoxin in dried dressed fillets of the puffer fish, Lagocephalus lunaris | 作者: | Tai-Yuan Chen Hsieh, Y. W. Tsai, Y. H. Shiau, C. Y. Deng-Fwu Hwang |
公開日期: | 十月-2002 | 出版社: | International Association for Food Protection | 卷: | 65 | 期: | 10 | 起(迄)頁: | 1670-1673 | 來源出版物: | Journal of Food Protection | 摘要: | The toxicity and species of dried dressed fish fillets were investigated. Three of seven dried dressed fish fillets were found to be toxic, with their levels of toxicity ranging from 9 to 18 mouse units per g. The toxin preparation was further purified and identified as tetrodotoxin and anhydrotetrodotoxin by high-performance liquid chromatography and gas chromatography–mass spectroscopy. The species of these fillets could not be distinguished from Lagocephalus lunaris by sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis or restriction site and sequence analysis of a polymerase chain reaction amplicon of the cytochrome b gene. These fish fillets were identified as L. lunaris on the basis of their protein patterns and gene characteristics. Furthermore, the toxic samples contained low levels of tetrodotoxin and its derivative. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/9749 | ISSN: | 0362-028X | DOI: | 10.4315/0362-028x-65.10.1670 |
顯示於: | 食品科學系 |
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