Effect of chenodeoxycholic acid on the toxicity of 5 alpha-cyprinol sulfate in rats

Abstract

Attempts are made to elucidate the effect of bile acid chenodeoxycholic acid on the toxicity of bile alcohol 5α-cyprinol in rats. Twenty-four male Wistar rats were divided into four groups and treated orally at 3-days periodic treatment with each 160 mg/kg of 5α-cyprinol sulfate/chenodeoxycholic acid (9:1), 5α-cyprinol sulfate and chenodeoxycholic acid for 19 days. After treated with 5α-cyprinol sulfate/chenodeoxycholic acid (9:1), 5α-cyprinol sulfate and chenodeoxycholic acid, the relative ratios of liver and kidney weight to body weight, the concentrations of red blood cells (RBC), hemoglobin and hematocrit in the blood, the levels of aspartate transferase (AST), alanine transferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine in the plasma, and the levels of BUN and creatinine in the urine of rats were significantly increased, but body weight of rats and the levels of Na+, K+, Ca++ in the urine of rats were significantly decreased, especially for both groups of 5α-cyprinol sulfate/chenodeoxycholic acid (9:1) and 5α-cyprinol sulfate. The pathological examination of liver and kidney also showed the cell enlargement and lesion in cell integrity in these treated groups, especially for both groups with 5α-cyprinol sulfate/chenodeoxycholic acid (9:1) and 5α-cyprinol sulfate. The toxicity of 5α-cyprinol sulfate/chenodeoxycholic acid (9:1) and 5α-cyprinol sulfate was similar to each other, and the toxic effect of chenodeoxycholic acid was less.