http://scholars.ntou.edu.tw/handle/123456789/15233
標題: | Identification of the small heat shock protein, HSP21, of shrimp Penaeus monodon and the gene expression of HSP21 is inactivated after white spot syndrome virus (WSSV) infection | 作者: | Po-Yu Huang Shih-Ting Kang Wei-Yu Chen Tai-Ching Hsu Chu-Fang Lo Kuan-Fu Liu Li-Li Chen |
關鍵字: | White spot syndrome virus (WSSV);Small heat shock protein (smHSP);penaeus monodon;Chaperone activity;Expressed sequence tag (EST) | 公開日期: | 九月-2008 | 出版社: | Elsevier | 卷: | 25 | 期: | 3 | 起(迄)頁: | 250-257 | 來源出版物: | Fish & Shellfish Immunology | 摘要: | The white spot syndrome virus (WSSV) is the causative agent of a severe disease in cultivated shrimp. The virus causes high mortality and leads to heavy stress on shrimps. In response to a variety of stresses, living organisms express particular sets of genes such as HSPs. In this study, a HSP21 gene, categorized into the small heat shock protein (smHSP) family, of shrimp Penaeus monodon was identified by annotating the EST databases established from WSSV-infected and WSSV-uninfected shrimp. The shrimp HSP21 gene was 555 bp in length. The thermal aggregation assay showed that the HSP21 had chaperone activity. The result of real-time PCR indicated that HSP21 was constitutive and inducible and was highly expressed in almost all organs such as the epithelium, gill, stomach, midgut, lymphoid organ, hepatopancreas, nervous tissue and heart, but less expressed in haemolymph. However, HSP21 gene showed down-regulation after WSSV infection. It suggests that gene regulation of HSP21 was seriously affected by WSSV. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/15233 | DOI: | https://doi.org/10.1016/j.fsi.2008.06.002 |
顯示於: | 海洋生物研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。