http://scholars.ntou.edu.tw/handle/123456789/17379
Title: | Carbonized nanogels for simultaneous antibacterial and antioxidant treatment of bacterial keratitis | Authors: | Lin, Hung-Yun Wang, Sin-Wen Mao, Ju-Yi Chang, Huan-Tsung Harroun, Scott G. Lin, Han-Jia Huang, Chih-Ching Lai, Jui-Yang |
Keywords: | Bacterial keratitis;Topical ocular administration;Carbonized nanogels;Anti-inflammatory;Bacteriostatic agents | Issue Date: | 1-May-2021 | Publisher: | ELSEVIER SCIENCE SA | Journal Volume: | 411 | Source: | CHEMICAL ENGINEERING JOURNAL | Abstract: | To prevent visual loss, clinical therapy of severe bacterial keratitis (BK) demands simultaneous remedying of the damage caused by bacterial infection and its induced oxidative stress/inflammation of the cornea. Here, we have developed a one-step method to synthesize carbonized nanogels (CNGs) from biogenic quercetin (Qu) and lysine (Lys) as a bifunctional agent with antibacterial and antioxidant properties for topical BK therapy. These Qu/LysCNGs synthesized by dry heating inherit and amplify the advantages of two natural products, including excellent antioxidant capacity and high positive charge. The Qu/Lys-CNGs display broad-spectrum bacteriostatic effects against non-multidrug-resistant bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, and also against multidrug-resistant bacteria, methicillin-resistant Staphylococcus aureus. Disintegration of peripheral structures of the bacteria caused by Qu/Lys-CNGs is due to their strong and specific interaction with bacterial membranes. In vitro cytotoxicity and hemolysis assays and in vivo corneal biocompatibility evaluations revealed good biocompatibility of Qu/Lys-CNGs as a safe nanomedicine in ophthalmic drop formulation. Furthermore, the Qu/Lys-CNGs can penetrate into the cornea via epithelial tight junction opening, thereby exerting superior antibacterial and antioxidant therapeutic effects against S. aureus-induced keratitis in rabbits. Our results indicate Qu/Lys-CNGs could be an effective bifunctional agent for clinical applications to treat bacterial ocular diseases. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/17379 | ISSN: | 1385-8947 | DOI: | 10.1016/j.cej.2021.128469 |
Appears in Collections: | 生命科學暨生物科技學系 |
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