http://scholars.ntou.edu.tw/handle/123456789/20413
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lin, Yu-Hsin | - |
dc.contributor.author | Chen, Chun-An | - |
dc.contributor.author | Tsai, Jenn-Shou | - |
dc.contributor.author | Chen, Guan-Wen | - |
dc.date.accessioned | 2022-02-17T03:53:30Z | - |
dc.date.available | 2022-02-17T03:53:30Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/20413 | - |
dc.description.abstract | This research focuses on cobia skin hydrolysates and their antihypertensive effects via the inhibitory activities of angiotensin I-converting enzyme (ACE). Marine fish Cobia (Rachycentron canadum) skin was hydrolysed for 5 h using Protamex and Protease N to obtain the cobia skin protein hydrolysates PX-5 and PN-5, respectively. The soluble protein and peptide contents of the PX-5 were 612 and 270 mg/g, respectively, and for the PN-5, 531 and 400 mg/g, respectively. The IC50 of PX-5 and PN-5 on ACE was 0.221 and 0.291 mg/mL, respectively. Increasing the IC50 from 0.221 to 0.044 mg/mL by simulated gastrointestinal digestion (PX-5G) reduced the ACE-inhibitory capacity of PX-5. Using gel filtration chromatography, the PX-5G was fractioned into eight fractions. The molecular weight of the fifth fraction from PX-5G was between 630 and 450 Da, and the highest inhibitory efficiency ratio on ACE was 1552.4%/mg/mL. We identified four peptide sequences: Trp-Ala-Ala, Ala-Trp-Trp, Ile-Trp-Trp, and Trp-Leu, with IC50 values for ACE of 118.50, 9.40, 0.51, and 26.80 mu M, respectively. At a dose of 600 mg PX-5 powder/kg body weight, in spontaneously hypertensive rats PX-5's antihypertensive effect significantly reduced systolic and diastolic blood pressure by 21.9 and 15.5 mm Hg, respectively, after 4 h of oral gavage. | - |
dc.language.iso | en_US | - |
dc.publisher | MDPI | - |
dc.relation.ispartof | NUTRIENTS | - |
dc.subject | ANGIOTENSIN-CONVERTING ENZYME | - |
dc.subject | ACE-INHIBITORY PEPTIDES | - |
dc.subject | PROTEIN HYDROLYSATE | - |
dc.subject | MUSCLE PROTEIN | - |
dc.subject | SPECTROPHOTOMETRIC ASSAY | - |
dc.subject | BLOOD-PRESSURE | - |
dc.subject | DARK MUSCLE | - |
dc.subject | PURIFICATION | - |
dc.subject | DIPEPTIDES | - |
dc.subject | FERMENTATION | - |
dc.title | Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates | - |
dc.type | journal article | - |
dc.identifier.doi | 10.3390/nu11061351 | - |
dc.identifier.isi | WOS:000474936700157 | - |
dc.identifier.url | <Go to ISI>://WOS:000474936700157 | - |
dc.relation.journalvolume | 11 | - |
dc.relation.journalissue | 6 | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.orcid | 0000-0002-3435-0725 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 食品科學系 03 GOOD HEALTH AND WELL-BEING 14 LIFE BELOW WATER |
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