http://scholars.ntou.edu.tw/handle/123456789/20470
標題: | Carbon black aggregates cause endothelial dysfunction by activating ROCK | 作者: | Yan, Junyan Lai, Chia-Hsiang Lung, Shih-Chun Candice Wang, Wen-Cheng Huang, Chih-Ching Chen, Guan-Wen Suo, Guangli Choug, Cheng-Tai Lin, Chia-Hua |
關鍵字: | TITANIUM-DIOXIDE NANOPARTICLES;DIESEL EXHAUST PARTICLES;RHO-KINASE;PROTEIN-KINASE;AIR-POLLUTION;AGGLOMERATION STATUS;ZNO NANOPARTICLES;VASCULAR-DISEASE;IN-VITRO;CELL | 公開日期: | 15-九月-2017 | 出版社: | ELSEVIER SCIENCE BV | 卷: | 338 | 起(迄)頁: | 66-75 | 來源出版物: | J HAZARD MATER | 摘要: | Carbon black nanoparticles (CBNs) have been associated with the progression of atherosclerosis. CBNs normally enter the bloodstream and crosslink together to form agglomerates. However, most studies have used nano-sized CB particles to clarify the involvement of CBN exposure in CBN-induced endothelial dysfunction. Herein, we studied endothelial toxicity of CBN aggregates (CBA) to human EA.hy926 vascular cells. Cell viability, lactate dehydrogenase leakage, and oxidative stress were affected by the highest concentration of CBA. Moreover, transmission electron microscopic results showed that CBA entered cells through membrane enclosed vesicles. Rho-associated kinase (ROCK) is involved in regulating vascular diseases. Thus, we co-treated with the of ROCK inhibitor Y-27632 to study whether other adverse effects caused by CBA are related to activating ROCK. As expected, co-treatment with Y-27632 attenuated CBA-induced cytoskeletal damage, dysfunction of the endothelial barrier, and expression of inflammatory factors. Taken together, these results demonstrate that aggregated CBNs can cause endothelial dysfunction possibly by activating ROCK. (C) 2017 Elsevier B.V. All rights reserved. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20470 | ISSN: | 0304-3894 | DOI: | 10.1016/j.jhazmat.2017.05.025 |
顯示於: | 生命科學暨生物科技學系 食品科學系 03 GOOD HEALTH AND WELL-BEING 11 SUSTAINABLE CITIES & COMMUNITIES |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。