http://scholars.ntou.edu.tw/handle/123456789/20539
標題: | DNA barcodes of the native ray-finned fishes in Taiwan | 作者: | Chang, Chia-Hao Kwang-Tsao Shao Lin, Han-Yang Chiu, Yung-Chieh Lee, Mao-Ying Liu, Shih-Hui Lin, Pai-Lei |
關鍵字: | FRESH-WATER-FISH;RIBOSOMAL-RNA GENE;SPECIES IDENTIFICATION;LEPTURUS PERCIFORMES;MARINE BIODIVERSITY;CLIMATE-CHANGE;PRODUCTS SOLD;ICHTHYOPLANKTON;AUTHENTICATION;REVEALS | 公開日期: | 七月-2017 | 出版社: | WILEY | 卷: | 17 | 期: | 4 | 來源出版物: | MOL ECOL RESOUR | 摘要: | Species identification based on the DNA sequence of a fragment of the cytochrome c oxidase subunit I gene in the mitochondrial genome, DNA barcoding, is widely applied to assist in sustainable exploitation of fish resources and the protection of fish biodiversity. The aim of this study was to establish a reliable barcoding reference database of the native ray-finned fishes in Taiwan. A total of 2993 individuals, belonging to 1245 species within 637 genera, 184 families and 29 orders of ray-finned fishes and representing approximately 40% of the recorded ray-finned fishes in Taiwan, were PCR amplified at the barcode region and bidirectionally sequenced. The mean length of the 2993 barcodes is 549 bp. Mean congeneric K2P distance (15.24%) is approximately 10-fold higher than the mean conspecific one (1.51%), but approximately 1.4-fold less than the mean genetic distance between families (20.80%). The Barcode Index Number (BIN) discordance report shows that 2993 specimens represent 1275 BINs and, among them, 86 BINs are singletons, 570 BINs are taxonomically concordant, and the other 619 BINs are taxonomically discordant. Barcode gap analysis also revealed that more than 90% of the collected fishes in this study can be discriminated by DNA barcoding. Overall, the barcoding reference database established by this study reveals the need for taxonomic revisions and voucher specimen rechecks, in addition to assisting in the management of Taiwan's fish resources and diversity. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20539 | ISSN: | 1755-098X | DOI: | 10.1111/1755-0998.12601 |
顯示於: | 海洋生物研究所 06 CLEAN WATER & SANITATION 13 CLIMATE ACTION 14 LIFE BELOW WATER 15 LIFE ON LAND |
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