http://scholars.ntou.edu.tw/handle/123456789/20746
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wu, Sheng-Han | - |
dc.contributor.author | Chou, Hsin-Yiu | - |
dc.contributor.author | Liu, Ping-Chung | - |
dc.contributor.author | Wu, Jen-Leih | - |
dc.contributor.author | Gong, Hong-Yi | - |
dc.date.accessioned | 2022-02-17T05:29:18Z | - |
dc.date.available | 2022-02-17T05:29:18Z | - |
dc.date.issued | 2019-08-6 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/20746 | - |
dc.description.abstract | In our previous study, the novel GRN-41 peptide generated from alternative splicing of the Mozambique tilapia PGRN1 gene was identified to be a potent peptide that protected against V. vulnificus in the transgenic zebrafish model by modulating innate immune-related genes. In this study, the anti-bacterial activities of synthetic Mozambique tilapia GRN-41 peptide (OmGRN-41) against various bacterial pathogens were investigated. The results showed that OmGRN-41 had bactericidal activity against Vibrio species, including V. vulnificus, V. alginolyticus, and V. harveyi, but exhibited bacteriostatic activity against V. parahaemolyticus. OmGRN-41 maintained bactericidal activity (64 mu M) against V. vulnificus at pH 2 to pH 10 or after heat treatment for 1 h at high temperatures between 40 degrees C and 100 degrees C. TEM observations revealed that the outer membrane of V. vulnificus was disrupted by OmGRN-41, leading to morphological rupture and loss of cytoplasmic contents. Additionally, little hemolytic activity against tilapia and sheep erythrocytes was detected after treatment with 128 mu M OmGRN-41. OmGRN-41 can effectively enhance the survival of Nile tilapia infected by V. vulnificus. Our results suggest that the OmGRN-41 is a novel antimicrobial peptide possessing bactericidal activity, especially against Vibrio species. These results indicate that OmGRN-41 can be applied in human Vibriosis treatment and has the potential to defend against Vibrio spp. infection in critical aquaculture organisms. (C) 2019 Elsevier Inc. All rights reserved. | - |
dc.language.iso | en_US | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.ispartof | BIOCHEM BIOPH RES CO | - |
dc.subject | HOST-DEFENSE | - |
dc.subject | GENE FAMILY | - |
dc.subject | ANTIBIOTICS | - |
dc.subject | ELASTASE | - |
dc.subject | INNATE | - |
dc.title | Granulin peptide GRN-41 of Mozambique tilapia is a novel antimicrobial peptide against Vibrio species | - |
dc.type | journal article | - |
dc.identifier.doi | 10.1016/j.bbrc.2019.06.022 | - |
dc.identifier.isi | WOS:000473557400028 | - |
dc.identifier.url | <Go to ISI>://WOS:000473557400028 | - |
dc.relation.journalvolume | 515 | - |
dc.relation.journalissue | 4 | - |
dc.relation.pages | 706-711 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.orcid | 0000-0002-6240-0582 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 水產養殖學系 14 LIFE BELOW WATER |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。