Effects of amylose content on starch-chitosan composite film and its application as a wound dressing

Abstract

This study analyzed the amylose content of starches and used them to prepare starch-chitosan composite films to assess their potential as wound dressings. Amylose content was 35.3% in potato starch, 30.5% in corn starch, and 9.7% in glutinous rice starch. The glutinous rice starch-chitosan composite (GC) film, which had a lower amylose content, had a coarser surface and exhibited a higher swelling rate, tensile strength, and elongation at break. In in vitro experiments by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that GC film had greater biocompatibility with mouse fibroblast L929 cells and human keratinocyte HaCaT cells. The results of the enzyme-linked immunosorbent assay indicated that GC film was more capable of alleviating inflammation than other films by preventing RAW264.7 macrophage from secreting cytokines (TNF- and IL-6). Additionally, it possessed an excellent coagulation rate. Briefly, the GC film with a lower amylose content is a suitable material for wound dressing.