http://scholars.ntou.edu.tw/handle/123456789/24445
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Tseng, Wen‐Chi | en_US |
dc.contributor.author | Jian, Li‐Min | en_US |
dc.contributor.author | Fang, Tsuei‐Yun | en_US |
dc.date.accessioned | 2024-01-19T06:47:50Z | - |
dc.date.available | 2024-01-19T06:47:50Z | - |
dc.date.issued | 2004-06 | - |
dc.identifier.issn | 0149-6395 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/24445 | - |
dc.description.abstract | Ions influence the separation resolution and elution profile obtained by ion-exchange chromatography. This study investigates the effects of buffer ions on the purity and yield of the plasmid purified by Q-Sepharose in a batch adsorption-desorption process when the wash buffers include either 30% or 45% isopropanol. When NaCl, KCl, or MgCl2 was used to remove RNA and proteins, the purified plasmid contained undetectable amounts of proteins and RNA at the salt concentrations that exceeded 1 M or 0.75 M in the presence of 30% and 45% isopropanol, respectively. Using CaCl2 and Na2SO4 led to the incomplete separation of plasmid from RNA. When applied to chromatographic columns packed with 20 or 200 mL of Q-Sepharose to purify plasmid from 300 and 3000 mL bacterial broth, respectively, stepwise elution of various concentrations of isopropanol and MgCl2 yielded a high quantity of plasmid with undetectable amounts of RNA and proteins. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | TAYLOR & FRANCIS | en_US |
dc.relation.ispartof | Separation Science and Technology | en_US |
dc.title | Effects of buffer ions on isopropanol-enhanced plasmid purification using Q-Sepharose | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1081/SS-120030769 | - |
dc.identifier.isi | WOS:000222685200004 | - |
dc.relation.journalvolume | 39 | en_US |
dc.relation.journalissue | 8 | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
item.openairetype | journal article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 食品科學系 |
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