Recovery of isoamylase from Pseudomonas amyloderamosa by adsorption-elution on raw starch

Abstract

The isoamylase from Pseudomonas amyloderamosa WU2130 was adsorbed onto raw starch, and the adsorbed enzyme was eluted by maltose in 50 mm acetate buffer. The adsorption of isoamylase to adsorbent was affected by the sources of raw starch, temperature, and pH, whereas the temperature and elution time had no obvious effects on the elution of adsorbed isoamylase. Raw starch could be used repeatedly on the adsorption-elution cycle with good reproducibility. About 52.5% of isoamylase was recovered, and the eluted enzyme had a specific activity of 72,828 U mg−1 protein with a purification of 13.3-fold. These results suggest that the raw starch adsorption-elution technique has a great potential in recovery of isoamylase from culture broths.