http://scholars.ntou.edu.tw/handle/123456789/9292
Title: | Accelerated bioethanol fermentation by using a novel yeast immobilization technique: Microtube array membrane | Authors: | Chien-Chung Chen Chien-Hui Wu Jhih-Jhong Wu Chien-Chih Chiu Chien-Hsuan Wong Min-Lang Tsai Hong-Ting Victor Lin |
Keywords: | Repeated-batch;Fermentation;Bioethanol;Microtube array membrane (MTAM);Kluyveromyces marxianus | Issue Date: | Oct-2015 | Publisher: | Elsevier | Journal Volume: | 50 | Journal Issue: | 10 | Start page/Pages: | 1509-1515 | Source: | Process Biochemistry | Abstract: | Cell immobilization is a way to isolate or localize intact cells in a certain space and maintain their catalytic activity. Immobilized cells can effectively reduce the negative effects of inhibitors and the processing cost of inoculum preparation for continuous or fed-batch fermentation of microorganisms. In this study, a novel yeast immobilization technique using renewable poly-l-lactic acid (PLLA) microtube array membrane (MTAM) was thoroughly evaluated for bioethanol fermentation. PLLA-MTAM was shown to be stable in 15% (v/v) ethanol solution during shaking cultivation. A yeast encapsulation efficiency of 67–70% was obtained, and the yeasts in MTAMs with greater porosity showed greater bioethanol productivity. The MTAM-immobilized Kluyveromyces marxianus, prepared using in situ and siphon methods, were evaluated using 5% (w/v) glucose fermentation. Improved glucose consumption and bioethanol production were observed in batch bioethanol fermentation. In 7 cycles during repeated-batch fermentation, the immobilized yeasts prepared using the in situ method showed a maximum CEtOH of 24.23 g/L, maximum YP/S of 0.48 g/g, and r PEtOH of 2.69 g/L h. Our data indicated that the PLLA-MTAM immobilized yeasts significantly enhanced bioethanol productivity and was a novel, promising technology for bioethanol fermentation. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/9292 | ISSN: | 1359-5113 | DOI: | 10.1016/j.procbio.2015.06.006 |
Appears in Collections: | 食品科學系 |
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