http://scholars.ntou.edu.tw/handle/123456789/20368
Title: | Improved Anticancer Photothermal Therapy Using the Bystander Effect Enhanced by Antiarrhythmic Peptide Conjugated Dopamine-Modified Reduced Graphene Oxide Nanocomposite | Authors: | Yu, Jiantao Lin, Yu-Hsin Yang, Lingyan Huang, Chih-Ching Chen, Liliang Wang, Wen-Cheng Chen, Guan-Wen Yan, Junyan Sawettanun, Saranta Lin, Chia-Hua |
Keywords: | CANCER-CELLS;GRAPHITE OXIDE;STEM-CELLS;REDUCTION;DELIVERY;NANOPARTICLES;POLYDOPAMINE;HYPERTHERMIA;GENOTOXICITY;COMPOSITES | Issue Date: | Jan-2017 | Publisher: | WILEY | Journal Volume: | 6 | Journal Issue: | 2 | Source: | ADV HEALTHC MATER | Abstract: | Despite tremendous efforts toward developing novel near-infrared (NIR)-absorbing nanomaterials, improvement in therapeutic efficiency remains a formidable challenge in photothermal cancer therapy. This study aims to synthesize a specific peptide conjugated polydopamine-modified reduced graphene oxide (pDA/rGO) nanocomposite that promotes the bystander effect to facilitate cancer treatment using NIR-activated photothermal therapy. To prepare a nanoplatform capable of promoting the bystander effect in cancer cells, we immobilized antiarrhythmic peptide 10 (AAP10) on the surface of dopamine-modified rGO (AAP10-pDA/rGO). Our AAP10-pDA/rGO could promote the bystander effect by increasing the expression of connexin 43 protein in MCF-7 breast-cancer cells. Because of its tremendous ability to absorb NIR absorption, AAP10-pDA/rGO offers a high photothermal effect under NIR irradiation. This leads to a massive death of MCF-7 cells via the bystander effect. Using tumor-bearing mice as the model, it is found that NIR radiation effectively ablates breast tumor in the presence of AAP10-pDA/rGO and inhibits tumor growth by approximate to 100%. Therefore, this research integrates the bystander and photothermal effects into a single nanoplatform in order to facilitate an efficient photothermal therapy. Furthermore, our AAP10-pDA/rGO, which exhibits both hyperthermia and the bystander effect, can prevent breast-cancer recurrence and, therefore, has great potential for future clinical and research applications. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20368 | ISSN: | 2192-2640 | DOI: | 10.1002/adhm.201600804 |
Appears in Collections: | 生命科學暨生物科技學系 食品科學系 03 GOOD HEALTH AND WELL-BEING |
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